През септември 2001 г. се омъжва за фотографа Сам Хандъл.<ref>{{cite web|url=http://www.samhandel.com/ |title=main |publisher=Samhandel.com |date= |accessdate=27 март 2013 |deadurl=yes |archive-urlarchiveurl=https://web.archive.org/web/20121230195610/http://www.samhandel.com/ |archive-datearchivedate=30 декември 2012 }}</ref> Семейството има две деца.<ref>{{cite web|url=http://www.people.com/people/article/0,,20009104,00.html|title=Six Feet Under's Lauren Ambrose Has a Boy|author=Alison Singh Gee|publisher=People}}</ref><ref>{{cite web|url=http://articles.latimes.com/2012/aug/25/entertainment/la-ca-lauren-ambrose-coma-20120826|title=Lauren Ambrose in fully engaged in 'Coma' on A&E|date=25 август 2012|author=T.L. Stanley|publisher=Los Angeles Times|accessdate=13 февруари 2013}}</ref><ref>{{cite web|url=http://www.indiewire.com/article/sleepwalk-with-me-star-lauren-ambrose-on-saying-goodbye-to-six-feet-under-and-playing-a-man-on-a-mission-in-a-e-medical-thriller-coma?page=1#articleHeaderPanel|title='Sleepwalk With Me' star Lauren Ambrose on saying goodbye to 'Six Feet Under' and why she took on A&E medical thriller 'Coma'|date=28 август 2012|author=Nigel M. Smith|publisher=Indiewire|accessdate=13 февруари 2013}}</ref>
